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Gen-Probe ltd
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Absolute Biotech
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Metabion International AG
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Digene Corp
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Microsynth ag
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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Magnetic Beads-Based Sensor with Tailored Sensitivity for Rapid and Single-Step Amperometric Determination of miRNAs
doi: 10.3390/ijms18112151
Figure Lengend Snippet: Schematic illustration of the fundamentals involved in the amperometric biosensor developed for the determination of microRNAs (miRNAs) based on the efficient formation of b-DNACp–miRNA heteroduplexes on the surface of Streptavidin-functionalized magnetic microcarriers (Strep-MBs) and their selective labeling with the anti-DNA–RNA hybrid antibody and ProtA–PolyHRP40. SPCE: screen-printed carbon electrode; b-DNACp: biotinylated complementary DNA probe.
Article Snippet: ProtA–PolyHRP40, a native ProtA labeled with a PolyHRP40, a homopolymer containing 40 HRP molecules covalently linked together (Catalog No.: ABIN929490, from antibodies-online) and an
Techniques: Labeling
Journal: International Journal of Molecular Sciences
Article Title: Magnetic Beads-Based Sensor with Tailored Sensitivity for Rapid and Single-Step Amperometric Determination of miRNAs
doi: 10.3390/ijms18112151
Figure Lengend Snippet: Optimization of the experimental variables affecting the performance of the amperometric biosensor developed for miRNA-21 determination.
Article Snippet: ProtA–PolyHRP40, a native ProtA labeled with a PolyHRP40, a homopolymer containing 40 HRP molecules covalently linked together (Catalog No.: ABIN929490, from antibodies-online) and an
Techniques: Incubation
Journal: International Journal of Molecular Sciences
Article Title: Magnetic Beads-Based Sensor with Tailored Sensitivity for Rapid and Single-Step Amperometric Determination of miRNAs
doi: 10.3390/ijms18112151
Figure Lengend Snippet: Working protocols evaluated to prepare the amperometric biosensor for the determination of miRNA-21.
Article Snippet: ProtA–PolyHRP40, a native ProtA labeled with a PolyHRP40, a homopolymer containing 40 HRP molecules covalently linked together (Catalog No.: ABIN929490, from antibodies-online) and an
Techniques: Hybridization, Labeling
Journal: International Journal of Molecular Sciences
Article Title: Magnetic Beads-Based Sensor with Tailored Sensitivity for Rapid and Single-Step Amperometric Determination of miRNAs
doi: 10.3390/ijms18112151
Figure Lengend Snippet: Dependence of the amperometric responses obtained with the developed biosensor in the absence (N) and in the presence of 100 pM (S) miRNA-21 and the resulting S/N current ratios, with the pre-incubation time of anti-DNA–RNA hybrid antibody and ProtA–PolyHRP40 mixture solution ( a ), and with the incubation time of b-DNACp-MBs with the mixture solution containing the target miRNA and the anti-DNA–RNA hybrid antibody preincubated for 1 h with the ProtA–PolyHRP40 ( b ). Error bars estimated at triple of the standard deviation of three replicates.
Article Snippet: ProtA–PolyHRP40, a native ProtA labeled with a PolyHRP40, a homopolymer containing 40 HRP molecules covalently linked together (Catalog No.: ABIN929490, from antibodies-online) and an
Techniques: Incubation, Standard Deviation
Journal: International Journal of Molecular Sciences
Article Title: Magnetic Beads-Based Sensor with Tailored Sensitivity for Rapid and Single-Step Amperometric Determination of miRNAs
doi: 10.3390/ijms18112151
Figure Lengend Snippet: Sensitivity obtained for different working conditions checked in the assessment of assay time shortening.
Article Snippet: ProtA–PolyHRP40, a native ProtA labeled with a PolyHRP40, a homopolymer containing 40 HRP molecules covalently linked together (Catalog No.: ABIN929490, from antibodies-online) and an
Techniques: Incubation